2′/3′‐<i>O</i>‐peptidyl Adenosine as a General Base Catalyst of its Own External Peptidyl Transfer: Implications for the Ribosome Catalytic Mechanism

Abstract

A physical organic study reveals general base catalysis by the 2′-oxyanion of the peptidyl adenosine ethanolysis; this implies a proton-shuttle role for the 2′-OH of peptidyl tRNA A76 in the ribosome substrate-assisted catalytic mechanism.