Publication | Closed Access
Cerulein-Induced Pancreatitis in Rats: Increased Lysosomal Enzyme Activity and Autophagocytosis
54
Citations
0
References
1985
Year
Supramaximal StimulationProtein SecretionImmunologyGastroenterologyPathologyGlycobiologyCytoskeletonCerulein-induced PancreatitisCellular PhysiologyGastrointestinal Peptide HormoneInflammationAutophagyExocrine PancreasSecretory PathwayBiochemistryEndocrine MechanismAutoimmunityEndocrinologyPharmacologyLysosomal Enzyme ActivityPhysiologyMetabolismMedicine
The involvement of lysosomes in the normal secretory process of the exocrine pancreas and in the onset of acute, hormone-induced pancreatitis was studied. The enzymatic activities of cathepsin B and beta-galactosidase were determined in the pancreas of rats that had been stimulated by either maximal (0.25 microgram X kg-1 h-1) or supramaximal (5 micrograms X kg-1 h-1) concentrations of cerulein. Maximal stimulation led to a moderate increase in cathepsin B activity and the ultrastructural appearance of multivesicular bodies. Supramaximal stimulation resulted in formation of large cytoplasmic vacuoles and progressive destruction of acinar cells which was paralleled by a marked increase of lysosomal enzyme activity.