Tritiated peptides. Part 3. Synthesis of [4-³H-Phe⁷]-β-corticotrophin-(1–24)-tetracosapeptide

Abstract

The synthesis is described of β-corticotrophin-(1–24)-tetracosapeptide labelled with tritium in the phenylalanine residue at position 7 to a specific radioactivity of 27 Ci mmol–1 by reductive deiodination of a protected precursor. Evidence for the integrity of the final product is provided by amino-acid analysis, column chromatography, and bioassay, supported by chemical and enzymic analytical data on the protected precursor and the derived free tetracosapeptide containing iodophenylalanine.