Serum Sulfhydryl Determinations and Significance in Connective Tissue Diseases

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Article1 September 1964Serum Sulfhydryl Determinations and Significance in Connective Tissue DiseasesARTHUR LORBER, M.D., CARL M. PEARSON, M.D., F.A.C.P., WROTHER L. MEREDITH, B.A., LOUISE E. GANTZ-MANDELL, B.A.ARTHUR LORBER, M.D.Search for more papers by this author, CARL M. PEARSON, M.D., F.A.C.P.Search for more papers by this author, WROTHER L. MEREDITH, B.A.Search for more papers by this author, LOUISE E. GANTZ-MANDELL, B.A.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-61-3-423 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptSulfhydryl (SH) groups are chemically reactive sites located on protein molecules. Oxidation of these groups results in the formation of disulfide bonds (S-S) (1), thereby interlinking the protein molecules and forming macroglobulins. These serum macroglobulins may be dissociated into their protein subunits by thiol (sulfhydryl-containing) compounds that have the ability to break the disulfide bonds (2-5). The importance of sulfhydryl groups in the formation of macroglobulins is further implied by the virtual absence of these groups in the abnormal protein found in Waldenstrom's macroglobulinemia (2) and by the decreased serum sulfhydryl levels reported in other related neoplastic disorders (6).Rheumatoid...References1. WERTHEIM E: Textbook of Organic Chemistry. The Blakiston Company, Philadelphia, 1945, p. 731. Google Scholar2. BLOCHPRASADANATASIBRIGGS HSAADR: Serum protein changes during administration of a low molecular weight thiol (penicillamine). J. Lab. Clin. Med. 56: 212, 1960. Google Scholar3. DEUTSCHMORTON HFJI: Dissociation of human serum macroglobulins. Science 125: 600, 1957. CrossrefMedlineGoogle Scholar4. DEUTSCHMORTON HFJI: Human serum macroglobulins and dissociation units. I. 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Author, Article, and Disclosure InformationAffiliations: Los Angeles, CaliforniaFrom the Departments of Medicine, Wadsworth Veterans Administration Hospital, Sepulveda Veterans Administration Hospital, and the University of California Medical Center, Los Angeles, Calif.This study was supported in part by a grant from the Southern California Chapter of the Arthritis and Rheumatism Association.Request for reprints should be addressed to Arthur Lorber, M.D., Veterans Administration Hospital, Sepulveda, Calif. 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