Effect of chlorpyrifos and monocrotophos on locomotor behaviour and acetylcholinesterase activity of subterranean termites, <i>Odontotermes obesus</i>

Abstract

The acute toxicity of chlorpyrifos and monocrotophos to subterranean termites, Odontotermes obesus (Rambur), has been studied by a paper contact method. The LC50 values for chlorpyrifos and monocrotophos were 0.046 and 0.148 microg cm(-2), respectively. Chlorpyrifos was 3.22-fold more toxic than monocrotophos. The effect of the pesticides on locomotor behaviour (velocity) and head acetylcholinesterase (AChE: EC 3.1.1.7) activity was estimated in LC50-exposed termites at intervals of 4, 8, 12, 16, 20 and 24 h. Chlorpyrifos- and monocrotophos-treated termites showed, respectively, 97 and 88% reduction in locomotor behaviour (velocity) after 24 h. At all time intervals the chlorpyrifos-treated termites exhibited more AChE inhibition and showed greater distorted behaviour than those exposed to monocrotophos. In vitro studies indicated that the I50 value (50% inhibition) for chlorpyrifos against AChE was 8.75 times that of monocrotophos.