Expression and purification of polyhistidine-tagged firefly luciferase in insect cells — a potential alternative for process scale-up

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251.6K citations

Life Sciences, Microbiology, Molecular Biology +14

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

Randall K. Saiki, Stephen J. Scharf, Fred Faloona et al. · Science · 1985 · 9K citations

Medicine, Genetics, Molecular Genetics +17

Metal chelate affinity chromatography, a new approach to protein fractionation

Jerker Porath, Jan Carlsson, Ingmar Olsson et al. · Nature · 1975 · 2.1K citations

Life Sciences, Biochemistry, Chemistry +14

A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures.

Summers, Gale Smith · 1987 · 1.7K citations

Life Sciences, Biology, Biotechnology +9

Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli

Verne A. Luckow, S.C. Lee, Gerard F. Barry et al. · Journal of Virology · 1993 · 911 citations · Full text

Life Sciences, Biotechnology, Molecular Biology +17