A Novel Amperometric Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase Incorporated in Organically Modified Sol-Gel Glass Matrix/Graphite Paste with Multiwalled Carbon Nanotubes

Abstract

Abstract We herein report an electrochemical hydrogen peroxide sensor based on horseradish peroxidase immobilized in organically modified sol-gel glass (ormosil) with mediator ferricyanide along with multiwalled carbon nanotubes (mwcnts). The ormosil material is converted to fine powder followed by incorporation within graphite paste electrode. The electrochemistry of redox materials encapsulated within ormosil has been studied. The requirement of mwcnts is examined. The ormosil prepared with optimum concentration of mwcnts shows better redox electrochemistry as compared to that made without mwcnts. The biosensor has been characterized by cyclic voltammetry and chroanoamperometry. The performance, stability, and reproducibility of a new peroxide biosensor are reported. Keywords: Amperometric sensorHorseradish peroxidase (HRP)Multiwalled carbon nanotubes (mwcnts)Ormosil modified electrodes The present research investigation has been funded by C.S.I.R., New Delhi Scheme no. 01(1983)/05/EMR-II. The author is highly grateful for this financial support. One of the authors, Manorma Singh, is grateful for the award of JRF from C.S.I.R., New Delhi.