The Role of an Essential Histidine Residue of Yeast Alcohol Dehydrogenase

Abstract

1 Inactivation of yeast alcohol dehydrogenase by diethyl pyrocarbonate indicates that one histidine residue per enzyme subunit is necessary for enzymic activity. The inactivated enzyme regains its activity over a period of days. 2 Enzyme modified by diethyl pyrocarbonate can form the binary enzyme · NADH complex with the same maximum NADH-binding capacity as that of native enzyme. Modified enzyme cannot form normal ternary complexes of the type enzyme · NADH · acetamide and enzyme · NAD+· pyrazole, which are characteristic of native enzyme. 3 The rate constant for the reaction of enzyme with diethyl pyrocarbonate has been determined over the pH range 5.5–9. The histidine residue involved has approximately the same pKa as free histidine, but is 10-fold more reactive than free histidine.