Hemagglutination by Measles Virus. 4. A Simple Procedure for Production of High Potency Antigen for Hemagglutination-Inhibition (HI) Tests

Abstract

A simple method for increasing hemagglutinating activity of measles virus tissue culture material is described. By treatment with Tween 80 and ether a 4- to 8-fold increase in titer was obtained with virus material grown in a human embryonic cell line (Lu 106) and an 8- to 32-fold increase when virus was grown in dog kidney TC. Maximal HA titers obtained with unconcentrated material were respectively 1:1024 and 1:4096 per 0.4 ml with the two different materials. Serum titers greater by a factor of 2 were obtained when Tween and ether treated material was substituted for untreated material as antigen in HI tests.