Nucleic Acids Research · 1985 · 16 citations · 20 references
Molecular BiologyAdenine MethytationThymidine Imino ProtonsNucleic Acid ChemistryProtein FoldingDna ComputingBiophysicsBiochemistryNmr StudyOligonucleotideDna ReplicationSolution Nmr SpectroscopyStructural BiologyRelaxation Time MeasurementsNatural SciencesProton TransferA-t Base PairSelective Nmr TechniquesProtein NmrMedicine
We report relaxation time measurements by semi-selective and totally selective NMR techniques on the thymidine imino protons of d(GGATATCC) and d(GGm6ATATCC). For these oligonucleotides helix fraying, rather than single base pair opening, is the major exchange mechanism even 25 degrees C below the Tm. We have therefore applied a new saturation transfer technique to measure exchange rates at temperatures where fraying has a very small or negligible contribution. Measurements of exchange rates as a function of temperature give significantly different activation energies for base pairs 3 and 4 in d(GGATATCC). Adenine methylation results in a slowing down of the opening rate for the m6A-T base pair but surprisingly has an even greater effect upon the adjacent non-methylated A-T base pair.
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