Science · 2008 · 181 citations · 19 references
Life SciencesCell BiologyMolecular BiologyMembrane BiophysicsMembrane TraffickingMolecular Cell BiologyLipid-protein InteractionsMembrane AsymmetryMembrane CurvatureMembrane ProteinsProtein-lipid InteractionsSecretory PathwayGolgi Apparatus MorphologyGolgi Apparatus StructureGolgi Membrane AsymmetryGolgi Transport VesiclesLipid Bilayer MembranesMembrane Curvature EffectsMembrane Curvature SensingVesicular Transport At The Golgi Apparatus
Golgins, long stringlike proteins, tether cisternae and transport vesicles at the Golgi apparatus. We examined the attachment of golgin GMAP-210 to lipid membranes. GMAP-210 connected highly curved liposomes to flatter ones. This asymmetric tethering relied on motifs that sensed membrane curvature both in the N terminus of GMAP-210 and in ArfGAP1, which controlled the interaction of the C terminus of GMAP-210 with the small guanine nucleotide-binding protein Arf1. Because membrane curvature constantly changes during vesicular trafficking, this mode of tethering suggests a way to maintain the Golgi architecture without compromising membrane flow.
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A human exchange factor for ARF contains Sec7- and pleckstrin-homology domains
Pierre Teilhard de Chardin, S Paris, Bruno Antonny et al. · Nature · 1996 · 465 citations