Suppression in vitro: Identification of a Serine-sRNA as a "Nonsense" Suppressor

Abstract

In a cell-free system, with RNA from a suppressible mutant of bacteriophage R17 as messenger,. no functional coat protein of the bacteriophage is synthesized unless serine-accepting soluble RNA from the suppressor strain, Escherichia coli S26RIE, is present.