Prostaglandin F_2α Receptor in Ovine <i>corpora lutea</i>

Abstract

Evidence is presented for the existence of a prostaglandin‐F 2α receptor in ovine corpora lutea. Prostaglandin F 2α was specifically bound to a particulate fraction of this tissue with a dissociation constant of 0.1μM. Prostaglandins F 1α , E 1 and E 2 had considerably less affinity for the receptor whereas prostaglandins A 2 and B 2 had little or no affinity. From a comparison of the binding constants of various prostaglandins and related compounds it could be concluded that the carboxyl group, the three hydroxyl groups and the 5,6‐ cis double bond (but not the 13,14‐ trans double bond) of prostaglandin F 2α were all important for binding to the receptor.