Ethylene Production and Plantlet Formation by<i>Nicotiana</i>Anthers Cultured in the Presence and Absence of Charcoal

Abstract

Anthers of Nicotiana tabacum produce ethylene when cultured for plantlet production. The rate is at a maximum 1–2 weeks after the onset of culture. Charcoal in the medium increases the proportion of androgenic anthers in N. tabacum and several other Nicotiana species. The level of ethylene in culture vessels is reduced by charcoal. However, complete removal of ethylene does not significantly alter the incidence of androgenesis, nor does continuous flushing of cultures with air. It is concluded that although charcoal reduces ethylene in the gas phase of the cultures its effect on androgenesis is exerted through some other mechanism.