Diblock Copolymer Micellar Nanoparticles Decorated with Annexin-A5 Proteins

Abstract

The formation of protein-decorated nanosized polymeric particles is described. Annexin-A5 protein binding to micellar aggregates having poly((1-ethoxycarbonyl)vinylphosphonic diacid) (PECVPD) and poly(n-butyl acrylate) (PBuA) as corona- and core-forming segments, respectively, was evidenced by Static and Dynamic Light Scattering (SDLS), cryo-Transmission Electron Microscopy (cryo-TEM), and Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) experiments. These new objects find potential applications, for example, in micelle-mediated target drug delivery and controlled fabrication of biochips.