Evidence that during very low density lipoprotein assembly in rat hepatocytes most of the triacylglycerol and phospholipid are packaged with apolipoprotein B in the Golgi complex

Abstract

Rat liver lipids were labelled by an intraportal injection of [3H]palmitic acid followed by isolation of rough and smooth endoplasmic reticulum or 'cis' or 'trans'-enriched Golgi fractions. The preparations were separated into membrane and contents and the apolipoprotein B of the content fractions was immunoprecipitated. More than 90% of the labelled triacylglycerol and phospholipid secreted into the blood immunoprecipitated with apolipoprotein B. Under the same experimental conditions 8, 12, 27 and 59% of the lipids of the rough, smooth, 'cis-Golgi' and 'trans-Golgi' contents, respectively, were immunoprecipitated. Thus, the 'trans-Golgi' region appears to be the major intracellular site of assembly of apolipoprotein B with triacylglycerol and phospholipid.