Real-Time Fluorescence Imaging of Isotachophoretic Preconcentration for Capillary Electrophoresis

Abstract

Real-time studies of the dynamic processes that take place during isotachophoresis (ITP) were performed. The experimental arrangement utilized was a real-time fluorescence imaging system based on a dye laser at 488 nm, pumped by a XeCl excimer laser. Fluorescence emitted from the migrating sample molecules was recorded by an image-intensified, thermoelectrically cooled CCD camera. The camera signals were processed by a computer and displayed on a screen in real time, allowing the ITP concentration to be monitored continuously. Real-time analysis provided additional information concerning ITP hardly obtainable using conventional detection systems or by theoretical calculations. Such experimental data can be evaluated and be compared with theoretical calculations. Information obtained by this detection technique is useful if ITP is to be used, for example as an on-line preconcentration technique in combination with capillary zone electrophoresis.