Proceedings of the National Academy of Sciences · 1978 · 141 citations · 14 references
Life SciencesBiochemistryMicrobiologyCellular MetabolismMetabolismMicrobial MetabolismAnaerobic MetabolismBacterial MetabolismCarbohydrate MetabolismCitric Acid CycleEnergy MetabolismTricarboxylic Acid CycleEscherichia ColiGlycolysisGlycolysis Under Anaerobic ConditionsGlycolytic PathwaysNuclear Magnetic Resonance SpectroscopyNuclear Magnetic Resonance StudiesPyruvate MetabolismValine Metabolism
High-resolution 13C nuclear magnetic resonance spectra of suspensions of Escherichia coli cells have been obtained at 90.5 MHz by using the Fourier transform mode. Anaerobic cells incubated with [I-13C]glucose show a time course of glycolysis in which the alpha and beta glucose anomers disappear at different rates, lactate, succinate, acetate, alanine, and valine accumulate as end products of glycolysis, and fructose bisphosphate appears as an intermediate. It is shown that fructose bisphosphate is labeled at C-1 and C-6 during [I-13C]-glucose catabolism. Upon oxygenation, glutamate appears with the 13C ENRICHMENT AT THE C-4, C-3, and C-2 positions, with the C-4 most intense. From the position of the 13C label we conclude that valine is formed by condensation of pyruvate and that carbon enters the tricarboxylic acid cycle mainly through acetyl CoA.
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Observation of tissue metabolites using 31P nuclear magnetic resonance
D. I. Hoult, Stephen Busby, David G. Gadian et al. · Nature · 1974 · 611 citations