Culture of fungi on cyanoacrylate skin surface strippings-a quantitative bioassay for evaluating antifungal drugs

Abstract

Current in vitro testing methods for antifungal drugs are often inadequate and sometimes correlate poorly with in vivo efficacy. We present a method of culture of fungi on cyanoacrylate skin surface strippings that can be used in combination with computerized image analysis to evaluate the efficacy of commercially available topical and oral antifungal drugs. Since this method closely copies the in vivo situation, it has obvious advantages over other in vitro investigative procedures and should be of relevance clinically.