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In Vitro Inducible Nitric Oxide Synthesis Inhibitors from Alismatis Rhizoma.

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19

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0

References

1999

Year

Nayoung Kim, Tai‐Hyun Kang, Hyun‐Ock Pae, Byung Min Choi, Hun‐Taeg Chung, Seung‐Woon Myung, Yun-Seon SONG, Dong-Hwan SHON, Youn‐Chul Kim
Biological and Pharmaceutical Bulletin

Abstract

Bioassay-guided fractionation of an aqueous extract of Alismatis Rhizoma has furnished two inducible nitric oxide synthase (iNOS) inhibitory compounds, alismol (1) and alisol B monoacetate (2), together with an inactive triterpene, alisol C monoacetate (3). Compounds 1 and 2 inhibited nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibitory effects of 1 and 2 on NO synthesis were partly due to suppression of iNOS mRNA expression as determined by Northern blotting.