In‐Situ Architectures Designed in 3D Cell‐Laden Hydrogels Using Microscopic Laser Photolithography

Abstract

Thermoresponsive bioactive hydrogels made from fibrinogen and Pluronic F127 are physically crosslinked at 37 °C to facilitate local chemical crosslinking using microscopic photolithography. The reaction conditions are mild enough to pattern in the presence of living cells. The interfaces between the chemically crosslinked “stiffer” and physically crosslinked “softer” regions capture the unique displays of morphogenesis in response to changing stiffness at the cellular scale. As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.