5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO): a new phosphorylated nitrone for the efficient In Vitro and In Vivo spin trapping of oxygen-centred radicals

Abstract

5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), a newly synthesized spin trap, is used for the in vitro spin trapping of hydroxyl and superoxide radicals, and though the spin trapping rates are close to those reported for 5,5-dimethyl-1-pyrroline N-oxide (DMPO), the DEPMPO–superoxide spin adduct is significantly more persistent than its DMPO analogue, a difference which allows the detection of superoxide during the reperfusion of ischaemic isolated rat hearts.