An Improved Histochemical Technic for Acid Phosphatase

Abstract

AbstractCauses of failure in the histochemical technic for acid phosphatase were studied. It was found that the ratio of buffer to substrate in the incubating mixture is an important factor in the constancy of results. In the case of glycerophosphate as a substrate, the optimal ratio is 5 to 6; with other substrates it may be different. The mechanism of the effect of this ratio on the results is explained.