Segmental Isotopic Labelling of a Multidomain Protein by Protein Ligation by Protein Trans‐Splicing

Abstract

Structure–function relationships of intact multidomain proteins: Segmental isotopic labelling of multidomain proteins could open a new avenue for studying particular domains in intact proteins, because it could simplify the NMR spectra through the incorporation of stable isotopes in a region-specific manner. We have demonstrated that the necessary protein ligation can be easily achieved both in vivo and in vitro by protein trans-splicing. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.