Purification and Properties of a Basic Endo-1,3-r -Glucanase from Rice (Oryza sativa L.)

Abstract

A 1,3-beta-glucanase purified from rice grain is a 33 kDa monomer with a pI of > or = 10.4. The enzyme was determined to be an endo-1,3-beta-glucanase (EC 3.2.1.39) by end product analysis using Laminaria digitata laminarin as substrate. Its amino-terminal amino acid sequence revealed strong homology to an endo-1,3-beta-glucanase from barley.