A Simple Synthesis of Sugar Nucleoside Diphosphates by Chemical Coupling in Water

Abstract

Sugar nucleotides made easy: The new reagent “ImIm”, which is formed in situ in water, is shown to activate nucleoside 5′-phosphates to their imidazolides, these can subsequently couple with sugar-1-phosphates; the whole procedure takes place in water. This truly simple method yields a crude product mixture that can be used directly as a source of donors for glycosyltransferase-mediated oligsaccharide synthesis. In the scheme, B stands for the nucleobases U, A, or G. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.