A Versatile Device for Microscopic Spectrofluorometry

Abstract

Abstract To facilitate a close study of nerve varicosities, cell fractions etc. by means of histochemical fluorescence techniques, an inexpensive microspectrofluorometer has been constructed. A fluorescence microscope with a strong light source is combined with a sensitive photometer and two motor‐driven grating monochromators. The equipment allows recording of excitation, emission and absorption spectra from areas about 1 μ in diameter.