Preparative Biochemistry & Biotechnology · 2006 · 21 citations · 31 references
Biochemical AnalysisRadiation EffectRat TissueRadiation ExposureRadiation BiologyReactive Oxygen SpeciesRedox BiologyProteomics AnalysisOxidative StressToxicologyOxidative Stress MarkersProteomicsRadiation OncologyHealth SciencesBiochemistryLiver PhysiologyIonizing RadiationReactive Oxygen SpecieRadiation EffectsCell BiologyReductive StressNon-ionizing RadiationPhysiologyMedicine
Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.
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