Glucose-Sensing Electrode Based on Carbon Paste Containing Ferrocene and Polyethylene Glycol-Modified Enzyme

Abstract

Abstract An amperometric glucose-sensing electrode is constructed by the use of a carbon paste containing 1,1′-dimethylferrocene and a polyethylene glycol-modified glucose oxidase. The polymer-modified enzyme is soluble in organic solvents (e. g., hexane) as well as in water, and exhibits a much higher activity than native enzyme does on the surface of a hydrophobic carbon paste eletrode. The higher enzyme activity results in an enhanced electrode response: the current response to glucose on the modified enzyme-based electrode is several times as large as that obtained on a native enzyme-based electrode.