Detection and subtyping of dengue 1–4 and yellow fever viruses by means of a multiplex RT‐nested‐PCR using degenerated primers

Abstract

This test is able to detect and identify the four DENVs and YFV to a high level of sensitivity and specificity and can be used with clinical samples. This simple, reliable and easy-to-perform method able to detect and identify dengue 1-4 and YFV can be used in poor endemic countries.