Nucleotide sequence of a 24,206-base-pair DNA fragment carrying the entire nitrogen fixation gene cluster of Klebsiella pneumoniae

[57] Sequencing end-labeled DNA with base-specific chemical cleavages

Allan M. Maxam, Walter Gilbert · Methods in enzymology on CD-ROM/Methods in enzymology · 1980 · 14.7K citations

Pharmacy, Biotechnology, Molecular Biology +13

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

Jeffrey Vieira, Joachim Messing · Gene · 1982 · 6.1K citations

Life Sciences, Genetics, Molecular Biology +15

A rapid boiling method for the preparation of bacterial plasmids

David S. Holmes, Michael E. Quigley · Analytical Biochemistry · 1981 · 3.3K citations

Life Sciences, Microbiology, Molecular Biology +13

Improved Estimation of Secondary Structure in Ribonucleic Acids

Ignacio Tinoco, Philip N. Borer, Barbara Dengler et al. · Nature New Biology · 1973 · 2.3K citations

Life Sciences, Biochemistry, Molecular Biology +14

A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria

Christopher F. Higgins, Ian D. Hiles, George P. C. Salmond et al. · Nature · 1986 · 763 citations

Life Sciences, Microbiology, Molecular Biology +14