Reproducibly High Plating Efficiencies of Isolated Mesophyll Protoplasts from Shoot Cultures of Tobacco

Abstract

Abstract Sterile shoot cultures of Nicotiana tabacum L. cv. Sansum proved to be a highly appropriate source for the isolation of viable protoplasts. High yields of isolated protoplasts and high plating efficiencies are guaranteed by the absence of contaminaction, by controlled culture conditions and by the rhythmic rejuvenation of the shoots at each transfer to fresh agar medium.