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Structure and subcellular localization of a small RNA‐binding protein from tobacco
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1997
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BiochemistryNatural SciencesRna Binding ProteinsRna Structure PredictionSubcellular LocalizationRna BiologyMolecular BiologySmall Rna-binding ProteinEscherichia ColiRelated ProteinSmall RnaSystems BiologyMedicineGene ExpressionRna ProcessingStructural BiologyProtein SynthesisSmall Rna‐binding Protein
In this study, a cDNA encoding a small RNA-binding protein was isolated from a Nicotiana sylvestris cDNA library. The predicted protein (RGP-3) is 144 amino acid residues long, and contains a consensus sequence-type RNA binding domain (CS-RBD) of 83 amino acids and a short glycine-rich region of 15 amino acids. RGP-3 synthesized in Escherichia coli has high affinity for poly(U). Immunocytochemical analysis indicated that RGP-3 is localized in the nucleoplasm, and that RGP-1b, a related protein reported previously, is localized in the nucleolus. Possible roles of these proteins in pre-mRNA or pre-rRNA processing are discussed.