Monoclonal antibodies against <i>Vibrio salmonicida</i>: the causative agent of coldwater vibriosis (‘Hitra disease’) in Atlantic salmon, <i>Salmo salar</i> L.

Abstract

Abstract. Monoclonal antibodies (Mab) directed against Vibrio salmonicida were produced and partially characterized. The bacterium is the causative agent of ‘Hitra disease’ or cotdwater vibriosis (CV) and differs from all other Vibrio bacteria tested so far with respect to a unique surface antigen (VS‐P1). Thirteen hybridoma clones produced antibodies which exclusively reacted with this antigen in ELISA. The remaining four clones reacted against undefined determinants and were partly cross‐reactive to V. anguiilarum, V. ordalii and V. fischeri . Fifteen Mab were of IgG1/kappa and two of the IgG3/kappa isotypes. Eleven of the IgG1 plus the two IgG3 Mab reacted with the VS‐P1 molecule.