Production of haploid Gerbera jamesonii plants by <i>in vitro</i> culture of unfertilized ovules

Abstract

In vitro culture, Ovule._ Haploid chlorophyllous plants have been successfully obtained in Gerbera jamesonii by in vitro culture of unfertilized ovules.After a callus had developed from the inside of the ovule, shoots differentiated.Several media were tested : the best results were noted on MURASHIGE and SKOOG's basic medium containing two cytokinins and one auxin (BAP 2 mg/l, kin 2 mg/l and IAA 0,5 mg/1).Shoots were cloned and rooted using the industrial method.Plants thus reconstituted were transferred to a greenhouse until flowering.As many as ten haploid plants per capitulum were obtained in this way.