The Journal of Clinical Endocrinology & Metabolism · 1973 · 30 citations · 0 references
MedicineEndocrinologyPediatricsPediatric EndocrinologyReproductive EndocrinologyReproductive MedicineEndocrine DiagnosticsEndocrine TestingHormonal EvaluationMale Reproductive EndocrinologyEndocrine AssaysGonadal Hormone AssaysGonadotropin PhysiologyHormone ImmunoassaysPrepubertal StatusReproductive Hormone AssaysUrinary GonadotropinsUrinary Hormone Measurement
FSH and LH were extracted by kaolin-acetone and acetone from pooled urine samples obtained from prepubertal children and normal men. Individual urine specimens obtained from 5 prepubertal boys receiving a short course of estrogen were also processed by the 2 methods. Gonadotropin content of the urinary concentrates was measured by radioimmunoassay. Physiologic changes at levels of FSH and LH far below the prepubertal range can be assessed by preparing urine specimens for radioimmunossay by either kaolin-acetone or acetone.