Construction of random small-insert genomic libraries highly enriched for simple sequence repeats

Abstract

Dinucleotide (CA)n repeat sequences are highly abundant and interspersed in eukaryotic genomes. Individual sites or loci can be identified by PCR-based assays using unique sequence oligonucleotides that flank specific CA-repeats. The number of CA-repeats at a given locus is variable making these markers highly informative for genetic analysis in humans (1) and other species (2). Unique sequences flanking specific (CA)n loci are usually identified by analyzing genomic libraries containing small size inserts, suitable for sequencing, generated by restriction enzymes. However the construction and screening of these type