Enzyme‐linked immunosorbent assay of 3‐acetyldeoxynivalenol applied to rice

Abstract

Abstract An enzyme‐linked immunosorbent assay for the detection of 3‐acetyldeoxynivalenol has been set up with an antiserum preparation raised against a bovine serum albumin—3‐acetyldeoxynivalenol hemiglutarate conjugate. The assay is an indirect, double antibody immunoassay performed on microtitration plates, and is sensitive (with a limit of detection of 1 pg per well) and specific. The assay has been validated for application to rice with a sensitivity of 1 ng g −1 . Sample extraction is a simple solubilisation procedure. The immunoassay described has a number of advantages over conventional methods of analysis.