Reciprocal inhibition between sensory ASH and ASI neurons modulates nociception and avoidance in Caenorhabditis elegans

Abstract

Sensory modulation is essential for animal sensations, behaviours and survival. Peripheral modulations of nociceptive sensations and aversive behaviours are poorly understood. Here we identify a biased cross-inhibitory neural circuit between ASH and ASI sensory neurons. This inhibition is essential to drive normal adaptive avoidance of a CuSO4 (Cu2+) challenge in Caenorhabditis elegans. In the circuit, ASHs respond to Cu2+ robustly and suppress ASIs via electro-synaptically exciting octopaminergic RIC interneurons, which release octopamine (OA), and neuroendocrinally inhibit ASI by acting on the SER-3 receptor. In addition, ASIs sense Cu2+ and permit a rapid onset of Cu2+-evoked responses in Cu2+-sensitive ADF neurons via neuropeptides possibly, to inhibit ASHs. ADFs function as interneurons to mediate ASI inhibition of ASHs by releasing serotonin (5-HT) that binds with the SER-5 receptor on ASHs. This elaborate modulation among sensory neurons via reciprocal inhibition fine-tunes the nociception and avoidance behaviour. In Caenorhabditis elegans, nociception and avoidance behaviour are crucial for survival, but their modulation is poorly understood. Here, the authors describe a cross-inhibitory neural circuit between ASH and ASI sensory neurons that fine-tunes nociception and mediates adaptive avoidance behaviour.