In vitro antibacterial potential of metal oxide nanoparticles against antibiotic resistant bacterial pathogens

Abstract

To investigate the antibacterial potential of 5 different metal oxide nanoparticles against antibiotic resistant bacterial pathogens viz., Pseudomonas aeruginosa, Klebsiella sp. Streptococcus pneumoniae, Staphylococcus aureus and Streptococcus sp. The antibacterial activity of the five different nanoparticles was assessed by well diffusion method. Different concentrations of the nanoparticles were analyzed by MIC and MBC techniques. Finally the potential MgO nanoparticle was also subjected for the time kill assay method. The results reveal that, the MgO nanoparticle showed maximum sensitivity [(16.00±0.53) mm dia] against Streptococcus pneumoniae and showed minimum sensitivity against Klebsiella sp. [(9.00±0.31) mm dia]. None of the nanoparticles showed sensitivity against the Streptococcus sp. The MIC result reveals that, the MgO nanoparticle showed maximum inhibition at a concentration of 10 μ g against Streptococcus pneumoniae. Moreover, the time kill assay reveals that, the bacterial growth was inhibited from the 2nd h onwards at a concentration of 10μ g. It is concluded from the present findings that, the MgO nanoparticle could be used as an alternative antibacterial agent after completing successful in vivo trials.