Determination of assay parameters for RNA analysis in bacterial and duodenal samples by spectrophotometry. Influence of sample treatment and preservation

Abstract

A simple analytical procedure derived from that described by Several procedures for sample preservation were tested ; the efficiency of each step of the analytical method was also determined using high performance liquid chromatography (HPLC). The proposed method (RNA eq) was compared with two other methods considered as references (2-6 diaminopimelic acid and 35 S incorporation) and microbial nitrogen flow was measured in defaunated sheep.