Physiologia Plantarum · 1990 · 26 citations · 17 references
Life SciencesPlant BiologyPlant BreedingEmbryo TransferPlant BiotechnologyPlant GeneticsPollen BiologyPollen DevelopmentPollen GerminationPollen ProductionPollen-pistil InteractionsPollination BiologyIn Vitro MaturationPollen CulturePollen EmbryologyPollen GrainsPollen Maturation
Microspores and young pollen grains can be isolated from the anther and matured in vitro. Pollination with in vitro matured pollen leads to seed set. This technique has potential applications in plant breeding. During in vitro maturation, the pollen can be used as a target for gene transfer.
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High-velocity microprojectiles for delivering nucleic acids into living cells
Theodore M. Klein, E. D. Wolf, Rong‐Ching Wu et al. · Nature · 1987 · 1.4K citations
Control of the developmental pathway of tobacco pollen in vitro
Masaharu Kyo, Hiroshi Harada · Planta · 1986 · 180 citations