The achaete-scute gene complex of D. melanogaster: Conserved Domains in a subset of genes required for neurogenesis and their homology to myc

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.3K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10

Detection of specific sequences among DNA fragments separated by gel electrophoresis

E.M. Southern · Journal of Molecular Biology · 1975 · 32.8K citations

Life Sciences, Molecular Biology, Molecular Genetics +12

Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I

Peter Rigby, Marianne Dieckmann, Carl Rhodes et al. · Journal of Molecular Biology · 1977 · 11.7K citations

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[2] New M13 vectors for cloning

Joachim Messing · Methods in enzymology on CD-ROM/Methods in enzymology · 1983 · 5.4K citations

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Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

Steven Henikoff · Gene · 1984 · 4.2K citations

Life Sciences, Genetics, Molecular Biology +5