THE CONFIGURATION OF GLYCOSIDIC LINKAGES IN OLIGOSACCHARIDES: III. <i>O</i>-α-<scp>D</scp>-MANNOPYRANOSYL-(1 → 2)-<i>O</i>-α-<scp>D</scp>-MANNOPYRANOSYL-(1 → 2)-<scp>D</scp>-MANNOSE

Abstract

A trisaccharide obtained after acetolysis of Saccharomycesrouxii mannan is shown to be O-α-D-mannopyranosyl-(1 → 2)-O-α-D-mannopyranosyl-(1 → 2)-D-mannose. Methylation and lead tetraacetate Oxidation techniques define the positions of the glycosidic linkages. The configurations of the two linkages are determined through use of two novel degradative methods which yield 2-O-α-D-mannopyranosyl-D-mannose and 2-O-α-D-mannopyranosyl-glycerol, each containing one of the glycosidic linkages of the parent trisaccharide.