Nucleotide sequence of a cDNA encoding ribosomal protein P0 in<i>Dictyostelium discoideum:</i>

Abstract

Ribosomal protein P0 is the eukaryotic equivalent to E. coli LlO through which L7/L12 bind to ribosomes (1). Results of a chemical cross-linking study made with Artemia ribosomes indicated that P0 forms a pentameric complex by interaction with dimers of P1 and P2 (2). It has been also reported that human P-proteins newly synthesized in vitro form similar pentameric complexes which were incorporated by preexisting 60S ribosomes (3). The primary structure of P0 from human ribosomes (3) and from Saccharomyces cerevisiae (4) have been aready determined, revealing that P0, P1 and P2 share nearly identical carboxyterminal sequences. This sequence contains an epitope which is recognized by autoantibodies in some patients suffering from systemic lupus erytiematosus (5).