DNA Fingerprinting to Examine Microgeographic Variation in the<i>Magnaporthe grisea</i>(<i>Pyricularia grisea</i>) Population in Two Rice Fields in Arkansas

Abstract

DNA fingerprinting was used to analyze microgeographic variation in the Magnaporthe grisea population in Arkansas. One hundred and thirteen isolates were collected from two commercial rice fields (cv. Newbonnet) in 1991. In addition, several reference isolates representing the predominant pathotypes in Arkansas were examined. Total DNA of each isolate was restricted with EcoR1 and probed with a dispersed repeated MGR586 DNA probe. MGR586 DNA fingerprint groups were identified based on DNA restriction fragment length polymorphism similarities. Seven distinct fingerprint groups (designated A through G) were identified among the field and reference isolates. These seven fingerprint groups were very similar to seven of the eight fingerprint groups (lineages) previously reported for M. grisea [...]