COMBINED APPROACH OF HEMATOLOGICAL BIOMARKERS AND PLASMA PROTEIN SAA FOR IMPROVEMENT OF RADIATION DOSE ASSESSMENT TRIAGE IN BIODOSIMETRY APPLICATIONS

Abstract

Early treatment of populations exposed to ionizing radiation requires accurate and rapid biodosimetry with a precision as high as possible to determine an individual's exposure level and risk for morbidity and mortality. The purpose of this study was to evaluate the utility of multiple blood biomarkers for early-response assessment of radiation exposure using a murine (BALB/c, males) in vivo radiation model. Present results for mice exposed to whole-body Co gamma-rays (0.1 Gy min) over a broad dose range (0-7 Gy) demonstrate at 24 h after exposure: 1) dose-dependent increase in the acute phase protein serum amyloid A or SAA; 2) dose-dependent changes in blood cell counts (lymphocytes, neutrophils, and ratio of neutrophils to lymphocytes); 3) SAA results coupled with peripheral blood cell counts analyzed with use of multivariate discriminant analysis established very successful separation of irradiated animals; 4) an enhanced separation as the number of biomarkers increased. These results also demonstrate proof-in-concept that plasma protein SAA shows promise as a complimentary approach to conventional biodosimetry for early assessment of radiation exposures and, coupled with peripheral blood cell counts, provides early diagnostic information to effectively manage radiation casualty incidents.