Journal of the American Chemical Society · 2009 · 96 citations · 32 references
Natural SciencesBiochemistryChemistryBiophotonicsMolecular ImagingNanomaterialsCellular ImagingFluorescence MicroscopyInorganic NanoparticlesNanomedicinePhotophysics Of Fluorescent ProbesQuantum Dots In BiologyCytotoxicity AssaysNanocrystalsNanoparticle Imaging ProbesSolvothermal SynthesisTwo-photon Excitation MicroscopyTwo-photon Fluorescence ImagingTwo-photon Fluorescence MicroscopyTwo-photon Microscopy
A novel sulfur-terminal Zn(II) complex Zn(S)(2)L (L = N-hexyl-3-{2-[4-(2,2':6',2''-terpyridin-4'-yl)phenyl] ethenyl}carbazole) was obtained by a facile solvothermal process. The unique feature in this new reaction design is the use of ZnS nanocrystals as a precursor and bulky chromophoric L as an ancillary ligand. The versatility of the two terminal sulfur atoms is relevant to biological system. The resulting Zn(S)(2)L complex shows two-photon excited fluorescence (TPEF), which has been proven to be potentially useful for two-photon microscopy imaging in living cells. In addition, cytotoxicity tests showed that the low-micromolar concentrations of Zn(S)(2)L did not cause significant reduction in cell viability over a period of at least 24 h and should be safe for further biological studies.
32
Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo
Daniel R. Larson, Warren R. Zipfel, Rebecca M. Williams et al. · Science · 2003 · 2.3K citations