Rapid Procedure for Estimating Cyclic Hydroxamate (DIMBOA) Concentration in Maize¹

Abstract

A rapid procedure was developed for estimating the concentration of a cyclic hydroxamic acid 2,4‐dihydroxy‐ 7‐methoxy‐l,4‐benzoxazin‐3‐one (DIMBOA) for potential use in a maize ( Zea mays L.) breeding program. Samples (0.2 to 0.5 g stem tissue) were collected from seedlings at 36 cm to 41 cm extended leaf height. The rapid procedure, essentially a simplification of the colorimetric procedure of Hamilton, was significantly correlated with the latter (r = +0.82) based on the analysis of 13 inbred lines of maize.