Annals of Tropical Medicine and Parasitology · 2002 · 69 citations · 15 references
Veterinary MedicineEntomologyInfectious DiseasesDiagnostic ParasitologyMedical MicrobiologyParasitologyHelminth DiagnosticsInfectious Disease Molecular TestingMolecular Diagnosis Of ParasitesPathogen DetectionSurveillance Of Parasitic DiseasesZoonotic Disease DiagnosisDna ExtractionLymphatic Filariasis EliminationMolecular Detection Of ParasitesMolecular Diagnosis Of Parasitic InfectionsMosquito-borne Disease DiagnosisParasitic DetectionPcr-based DetectionRapid Diagnostic Assays
PCR has recently been studied as a promising tool for monitoring the progress of efforts to eliminate lymphatic filariasis. PCR can be used to test concurrently at least 30 pools, with as many as 40 mosquitoes in each pool, for the presence of filarial larvae. The SspI PCR assay for the detection of Wuchereria bancrofti DNA in pools of mosquitoes has been used since 1994 in a variety of laboratories worldwide. During that time, the original assay has been modified in these different laboratories and no standardized assay currently exists. In an effort to standardize and improve the assay, a meeting was held on 15-16 November 2001, at Emory University in Atlanta, with representatives from most of the laboratories currently using the assay. The first round of testing was designed to test the four most promising methods for DNA extraction from pools of mosquitoes. Two of the four methods stood out as clearly the best and these will be now optimised and evaluated in two further rounds of testing.
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Reda M. R. Ramzy, Hoda A. Farid, Ibrahim H. Kamal et al. · Transactions of the Royal Society of Tropical Medicine and Hygiene · 1997 · 107 citations